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We
are currently recommending Agilent
oligo arrays for human, mouse, or rat samples. We
also offer cDNA arrays from Agilent or the University
of Miami for human samples. For more specialized needs
ask about our custom arrays, which are fabricated
to your specifications. Descriptions for Agilent
and UM arrays follow below:
Agilent
Arrays
Human
1A Oligo Array | Human
1B Oligo Array | Whole Human Genome Array | Mouse
Development Oligo Array | Mouse
Oligo Array | Whole Mouse Genome Array | Rat Oligo
Array | Whole Rat Genome Array |Yeast Oligo
Array | Agilent Custom
Arrays
Human
1A Oligo Array:
-
Gene list available here
-
Microarray Format: 22K
- Probe
Type: in situ
- Probe
Length: 60 bases
- Probes/Microarray:
22,575
- Average
Accuracy: Within 1.4 fold of expected target
ratio
- Manufacturing
process: in situ oligo synthesis
-
Feature size: 135 microns
-
Detection Limits: 1 transcript/cell/million
cells
-
Average Reproducibility: Median Std.Dev. (log
ratio) 0.015
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Dynamic Range: 0.05 - 5.00 pM
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Human
1B Oligo Array:
-
Gene list available here
-
Microarray Format: 22K
- Probe
Type: in situ
- Probe
Length: 60 bases
- Probes/Microarray:
22,575
- Manufacturing
process: in situ oligo synthesis
-
Feature size: 135 microns
-
Detection Limits: 1 transcript/cell/million
cells
-
Average Reproducibility: Median Std.Dev.<0.018
-
Dynamic Range: 0.05 - 5.00 pM
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Whole Human Genome Array:
- Gene list available here
- Microarray Format: 44K (double density)
- Probe Type: in situ
- Probe Length: 60 bases
- Probes/Microarray: ca. 41,000
- Average Accuracy: Within 1.4 fold of expected target ratio
- Manufacturing process: in situ oligo synthesis
- Feature size: 110 microns
- Detection Limits: 1 transcript/cell/million cells
- Average Reproducibility: Median Std.Dev. < 0.013
- Dynamic Range: 0.05 - 5.00 pM
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Mouse
Development Oligo Array:
- Gene list available here
- Microarray
Format: 22K
- Probe
Type: in situ
- Probe
Length: 60 bases
- Probes/Microarray:
20,371
- Average
Accuracy: Within 1.4 fold of expected target
ratio
- Manufacturing
process: in situ oligo synthesis
- Feature
size: 135 microns
- Detection
Limits: 1 transcript/cell/million cells
- Average
Reproducibility: Median Std.Dev. (log ratio)
0.015
- Dynamic
Range: 0.05 - 5.00 pM
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Mouse
Oligo Array:
- Gene list available here
- Microarray Format: 22K
- Probe Type: in situ
- Probe Length: 60 bases
- Probes/Microarray: 20,371
- Average Accuracy: Within 1.4 fold of expected target ratio
- Manufacturing process: in situ oligo synthesis
- Feature size: 135 microns
- Detection Limits: 1 transcript/cell/million cells
- Average Reproducibility: Median Std.Dev. (log ratio) 0.015
- Dynamic Range: 0.05 - 5.00 pM
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Whole Mouse Genome Array:
- Gene list available here
- Microarray Format: 44K (double density)
- Probe Type: in situ
- Probe Length: 60 bases
- Probes/Microarray: ca. 41,000
- Average Accuracy: Within 1.4 fold of expected target ratio
- Manufacturing process: in situ oligo synthesis
- Feature size: 110 microns
- Detection Limits: 1 transcript/cell/million cells
- Average Reproducibility: Median Std.Dev. < 0.013
- Dynamic Range: 0.05 - 5.00 pM
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Rat
Oligo Array:
- Gene list available here
- Microarray
Format: 22K
- Probe
Type: in situ
- Probe
Length: 60 bases
- Content
Source: National Institute of Environmental
Health Sciences,
and Incyte Genomics Zooseq Database, RefSeq,
GenBank
- Genes/Microarray:
~20,000
- Probes/Microarray:
22,575
- Average
Accuracy: Within 1.4 fold of expected target
ratio
- Manufacturing
process: in situ oligo synthesis
- Feature
size: 135 microns
- Detection
Limits: 1 transcript/cell/million cells
- Average
Reproducibility: Median Std.Dev. (log ratio)
0.015
- Dynamic
Range: 0.05 - 5.00 pM
TOP
Whole Rat Genome Array:
- Gene list available here
- Microarray Format: 44K (double density)
- Probe Type: in situ
- Probe Length: 60 bases
- Probes/Microarray: ca. 41,000
- Average Accuracy: Within 1.4 fold of expected target ratio
- Manufacturing process: in situ oligo synthesis
- Feature size: 110 microns
- Detection Limits: 1 transcript/cell/million cells
- Average Reproducibility: Median Std.Dev. < 0.013
- Dynamic Range: 0.05 - 5.00 pM
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Yeast
Oligo Array:
- Gene list available here
- Microarray
Format: 11K
- Probe
Type: in situ
- Probe
Length: 60 bases
- Content
Source: Saccharomyces Genome Database 25-May-2002
ORF list
- Genes/Microarray:
6,256 ORFs
- Probes/Microarray:
10,267
- Average
Accuracy: Within 1.4 fold of expected target
ratio
- Manufacturing
process: in situ oligo synthesis
- Feature
size: 135 microns
- Detection
Limits: 1 transcript/cell/million cells
- Average
Reproducibility: Median Std.Dev. (log ratio)
0.015
- Dynamic
Range: 0.05 - 5.00 pM
TOP
Below:
Close-up of Agilent's whole human genome array
showing the double-density format, which can
accommodate 44,000 features on a single 1"
x 3" glass-slide.
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Agilent
Custom Arrays:
60-mer
Oligonucleotide Microarrays - These extended-length
microarrays are custom designed to meet your
needs. Three probe density format configurations
are available: 1,100 features per array (8 arrays/slide) or 8,455 features per array (2
arrays/1" x 3" glass slide) or 22,575
features per array (1 array/slide).
Visit
the Agilent Web
site for more information.
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UM
Arrays
Gene
lists are available for the following UM arrays:
UM
Human cDNA Array (Excel ~1.4MB)
UM
Mouse Oligo Array
(Zipped Excel File, ~2MB)
For
more information or to inquire about UM Custom Arrays,
please contact Dr.
Lubov Nathanson.
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