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ELECTRON MICROSCOPY CORE FACILITY
SERVICES AND EQUIPMENT AVAILABLE:
The primary purpose of the EM Core is to provide the University of Miami, Miller School of Medicine academic and research community access to the conventional transmission and scanning electron microscopy capabilities.
Website
Contact:
Anna Gomez
305-243-9774
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PHOSPHOIMAGER
CORE FACILITY
Contact:
Mary Lou King, Ph.D.
(Raja) 305-243-5628
Gautier Building; Room 515
1600 NW, 10th Ave. Miami, Fl.
DESCRIPTION OF FACILITY:
Storage phosphor autoradiography system (Storm 840 PhosphorImager
from
Molecular Dynamics) for imaging and analysis of radioactive samples.
The
scanner accepts standard 20X25 cm format storage phosphor screens.
The core facility has 10 screens available for common use. The system
includes the
ImageQuaNT data analysis software for use with Windows NT. The software
will
allow the investigator to quantitate radioactive samples in a linear
fashion
over five logs. Film is linear only over differences of three-fold.
The
phosphor system reduces exposure times to at least half the time
required by
standard x-ray film. Screens are sensitive to 32P,35S, and 125I
radioisotopes.
Also
available is the Chemilmager 5500 Fluorescence, Chemiluminescence,
and
Visible Imaging System from Alpha Innotech Corporation. Currently
we have a
UV transilluminator and filters for EtBr and SYBR green staining
as well as
a filter for Rhode Island Red. Useful for Western blots and RNA/DNA
and
protein quantitation. Software for quantitation is available.
SERVICES
AVAILABLE:
Training in the proper use of the screens and the software programs
are
available for full data analysis.
SERVICE
CHARGES:
$5.00 per screen usage up to three days exposure time.
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