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TRANSMISSION
AND SCANNING ELECTRON MICROSCOPES
Contact:
Anna Gomez
305-243-6982
Rosenstiel Medical Sciences Building; Room 4119
1600 NW, 10th Avenue Miami, Florida
SERVICES AND EQUIPMENT AVAILABLE:
The Department of Cell biology and Anatomy has a complete biological
electron microscopy facility available to any student, faculty member,
researcher, or corporate entity who may need these specialized techniques.
Ancillary to the JEOL CX-100 TEM and JEOL 35 SEM we have Porter/Blum
Ultramicrotomes, a Sorvall critical pint dryer, a Hummer gold sputter
coater, Polaroid MP-3 and MP-4 copystands, and negative/print photographic
processing darkrooms with Devere and Omega enlargers. We are the
only core EM facility on the Medical School Campus that allows their
equipment to be accessed by people outside the department. To help
defray the cost of equipment, expendables, service contracts, and
salary expenses we operate on a "for fee" basis. These
moneys usually recover 50-60% of the yearly operational costs. We
also participate in an electron microscopy course taught each fall
on the main campus to undergraduate students.
USERS
AND APPLICATIONS:
Our microscopes have been used at one time or another by nearly
every Department on the Medical School Campus. We have also done
projects for the biology and material science departments on the
Coral Gables Campus. In addition, we have been utilized by Coulter,
Cordis, Ad-Tech, the Historical Museum of South Florida, local high
schools for demonstrations to students, and private consulting firms
needing micrographs of their client's products of projects. We cater
to a wide diversity of projects such as: cultured cells exposed
to antibiotics or chemical agents to show the structural changes;
fertilization and developmental biology ultrastructure; pre-and
post- transplant organ analysis and diagnosis; RBC parasites and
abnormalities; wound healing in skin grafts; nerve regeneration
in culture chambers; dermatological parasites of various sorts;
forensic analysis of hair and fibers; comparative and human pathology
of tumors, viruses, and parasites; intraocular lenses, arterial
stents, and catheter tips used in surgical implants/ procedures-
to list but a few of the many studies we have done.
SERVICE
CHARGES:
A.
Inside Cell Biology/Anatomy (Beam Time only) =$25.00/hour
B. Inside Cell Biology/Anatomy (Technical Time) =$50.00/hour
C. Adjunct Faculty =$50.00/hour
D. All outside Cell Biology /Anatomy use =$100.00/hour
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PHOSPHOIMAGER
CORE FACILITY
Contact:
Mary Lou King, Ph.D.
(Raja) 305-243-5628
Gautier Building; Room 515
1600 NW, 10th Ave. Miami, Fl.
DESCRIPTION OF FACILITY:
Storage phosphor autoradiography system (Storm 840 PhosphorImager
from
Molecular Dynamics) for imaging and analysis of radioactive samples.
The
scanner accepts standard 20X25 cm format storage phosphor screens.
The core facility has 10 screens available for common use. The system
includes the
ImageQuaNT data analysis software for use with Windows NT. The software
will
allow the investigator to quantitate radioactive samples in a linear
fashion
over five logs. Film is linear only over differences of three-fold.
The
phosphor system reduces exposure times to at least half the time
required by
standard x-ray film. Screens are sensitive to 32P,35S, and 125I
radioisotopes.
Also
available is the Chemilmager 5500 Fluorescence, Chemiluminescence,
and
Visible Imaging System from Alpha Innotech Corporation. Currently
we have a
UV transilluminator and filters for EtBr and SYBR green staining
as well as
a filter for Rhode Island Red. Useful for Western blots and RNA/DNA
and
protein quantitation. Software for quantitation is available.
SERVICES
AVAILABLE:
Training in the proper use of the screens and the software programs
are
available for full data analysis.
SERVICE
CHARGES:
$5.00 per screen usage up to three days exposure time.
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