Research Interests
My research focuses on the initiation of inflammation, autophagy and apoptosis in the CNS  after traumatic brain and spinal cord injury. Our results demonstrate that cell death processes after CNS injury involve both the activation of multiple caspases in the apoptotic pathway and initiation of inflammatory caspase-activating platforms. Moreover, CNS injury also involves activation of the autophagy signaling pathway. Our current work seeks to understand the physiological functions of these signaling pathways after CNS injury that may provide promising and unique therapeutic strategies applicable to the treatment of CNS injury and disease.
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Cortical neurons express TNF receptor 1 (TNFR1) and the lipid raft marker, flotillin-1 at 15 minutes after moderate traumatic brain injury. The high-resolution confocal image shows that TNFR1 is present in lipid raft microdomains in the plasma membrane surrounding the cell soma and in cell processes. Sections were triple-stained with NeuN (blue), TNFR1 (green) and flotillin-1 (red). For details, see the article by Lotocki et al. 2004. |
Fall 2005 Members of the Keane Lab
From Left to Right:
Gregory Bigford
Angela Davis
Robert W. Keane
Juan Pablo de Rivero Vaccari |
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Selected Publications
- Keane RW, Davis AR, Dietrich WD (2005) Inflammatory and apoptotic signaling following spinal cord injury. J. Neurotrauma (in press).
- Lotocki G, Alonso OF, Dietrich WD, Keane RW (2004) Tumor necrosis factor receptor 1 and its signaling intermediates are recruited to lipid rafts in the traumatized brain. J Neurosci. 24(49):11010-6.
- Lotocki G, Alonso OF, Frydel B, Dietriech WD, Keane RW (2003) Monoubiquitination and cellular redistribution of XIAP in neurons after traumatic brain injury. J Cereb Blood Flow Metabolism 23:1129-1136.
- Lotocki G and Keane RW (2002) Inhibitors of apoptosis in injury and disease. IUBMB Life 54(5):231-40.
- Ozawa H, Keane RW, Marcillo AE, Diaz PH, Dietrich WD (2002) Therapeutic strategies targeting caspase inhibition following spinal cord injury in rats. Exp Neurol. 2002 Sep;177(1):306-13.
- Katz, LM, Lotocki G, Wang Y, Kraydieh S, Dietrich WD, Keane RW (2001) Regulation of caspases and XIAP after asphyxial cardiac arrest in rats. Neuroreport 12:3751-3754.
- Keane RW, Kraydieh S, Lotocki G, Aldana P, Dietrich WD (2001) Apoptotic and anti-apoptotic mechanisms following traumatic brain injury. J. Cereb. Blood Flow and Metab. 21:1189-1198.
- Keane RW, Kraydieh S, Lotocki G, Bethea, JR, Krajewski S, Reed JC and Dietrich WD (2001) Apoptotic and anti-apoptotic mechanisms following spinal cord injury. J. Neuropathol. Exp. Neurol. 60:422-429.
Curriculum Vitae
- 1968
B.A. State University of New York, Buffalo
- 1971
M.S. University of California at Davis
- 1976
Ph.D. Developmental Genetics, University of California at Davis
- 1977-1980
Postdoctoral Fellow, Molecular Biology/Developmental
Neurobiology, Massachussetts Institute of Technology
- 1980-1982
Assistant Professor, Rutgers University
- 1983-1989
Assistant Professor, Dept. Physiology and Biophysics,
University of Miami School of Medicine, Miami, FL
- 1989-present
Associate Professor, Dept. Physiology and Biophysics,
University of Miami School of Medicine, Miami, FL
- 1995-1996
Visiting Professor, Burnham Institute, La Jolla CA
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